Transcriptome alteration in Phytophthora infestans in response to phenazine-1-carboxylic acid production by Pseudomonas fluorescens strain LBUM223.
Identifieur interne : 000633 ( Main/Exploration ); précédent : 000632; suivant : 000634Transcriptome alteration in Phytophthora infestans in response to phenazine-1-carboxylic acid production by Pseudomonas fluorescens strain LBUM223.
Auteurs : Roxane Roquigny [Canada] ; Amy Novinscak [Canada] ; Tanya Arseneault [Canada] ; David L. Joly [Canada] ; Martin Filion [Canada]Source :
- BMC genomics [ 1471-2164 ] ; 2018.
Descripteurs français
- KwdFr :
- Agents de lutte biologique (MeSH), Analyse de profil d'expression de gènes (MeSH), Analyse de séquence d'ARN (MeSH), Phytophthora infestans (croissance et développement), Phytophthora infestans (génétique), Phytophthora infestans (métabolisme), Phénazines (métabolisme), Pseudomonas fluorescens (métabolisme), Transcriptome (MeSH).
- MESH :
- croissance et développement : Phytophthora infestans.
- génétique : Phytophthora infestans.
- métabolisme : Phytophthora infestans, Phénazines, Pseudomonas fluorescens.
- Agents de lutte biologique, Analyse de profil d'expression de gènes, Analyse de séquence d'ARN, Transcriptome.
English descriptors
- KwdEn :
- Biological Control Agents (MeSH), Gene Expression Profiling (MeSH), Phenazines (metabolism), Phytophthora infestans (genetics), Phytophthora infestans (growth & development), Phytophthora infestans (metabolism), Pseudomonas fluorescens (metabolism), Sequence Analysis, RNA (MeSH), Transcriptome (MeSH).
- MESH :
- chemical , metabolism : Phenazines.
- chemical : Biological Control Agents.
- genetics : Phytophthora infestans.
- growth & development : Phytophthora infestans.
- metabolism : Phytophthora infestans, Pseudomonas fluorescens.
- Gene Expression Profiling, Sequence Analysis, RNA, Transcriptome.
Abstract
BACKGROUND
Phytophthora infestans is responsible for late blight, one of the most important potato diseases. Phenazine-1-carboxylic acid (PCA)-producing Pseudomonas fluorescens strain LBUM223 isolated in our laboratory shows biocontrol potential against various plant pathogens. To characterize the effect of LBUM223 on the transcriptome of P. infestans, we conducted an in vitro time-course study. Confrontational assay was performed using P. infestans inoculated alone (control) or with LBUM223, its phzC- isogenic mutant (not producing PCA), or exogenically applied PCA. Destructive sampling was performed at 6, 9 and 12 days and the transcriptome of P. infestans was analysed using RNA-Seq. The expression of a subset of differentially expressed genes was validated by RT-qPCR.
RESULTS
Both LBUM223 and exogenically applied PCA significantly repressed P. infestans' growth at all times. Compared to the control treatment, transcriptomic analyses showed that the percentages of all P. infestans' genes significantly altered by LBUM223 and exogenically applied PCA increased as time progressed, from 50 to 61% and from to 32 to 46%, respectively. When applying an absolute cut-off value of 3 fold change or more for all three harvesting times, 207 genes were found significantly differentially expressed by PCA, either produced by LBUM223 or exogenically applied. Gene ontology analysis revealed that both treatments altered the expression of key functional genes involved in major functions like phosphorylation mechanisms, transmembrane transport and oxidoreduction activities. Interestingly, even though no host plant tissue was present in the in vitro system, PCA also led to the overexpression of several genes encoding effectors. The mutant only slightly repressed P. infestans' growth and barely altered its transcriptome.
CONCLUSIONS
Our study suggests that PCA is involved in P. infestans' growth repression and led to important transcriptomic changes by both up- and down-regulating gene expression in P. infestans over time. Different metabolic functions were altered and many effectors were found to be upregulated, suggesting their implication in biocontrol.
DOI: 10.1186/s12864-018-4852-1
PubMed: 29914352
PubMed Central: PMC6006673
Affiliations:
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Joly</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, Université de Moncton, Moncton, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, Université de Moncton, Moncton</wicri:regionArea><wicri:noRegion>Moncton</wicri:noRegion></affiliation></author><author><name sortKey="Filion, Martin" sort="Filion, Martin" uniqKey="Filion M" first="Martin" last="Filion">Martin Filion</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, Université de Moncton, Moncton, Canada. martin.filion@umoncton.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, Université de Moncton, Moncton</wicri:regionArea><wicri:noRegion>Moncton</wicri:noRegion></affiliation></author></analytic><series><title level="j">BMC genomics</title><idno type="eISSN">1471-2164</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biological Control Agents (MeSH)</term><term>Gene Expression Profiling (MeSH)</term><term>Phenazines (metabolism)</term><term>Phytophthora infestans (genetics)</term><term>Phytophthora infestans (growth & development)</term><term>Phytophthora infestans (metabolism)</term><term>Pseudomonas fluorescens (metabolism)</term><term>Sequence Analysis, RNA (MeSH)</term><term>Transcriptome (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agents de lutte biologique (MeSH)</term><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Analyse de séquence d'ARN (MeSH)</term><term>Phytophthora infestans (croissance et développement)</term><term>Phytophthora infestans (génétique)</term><term>Phytophthora infestans (métabolisme)</term><term>Phénazines (métabolisme)</term><term>Pseudomonas fluorescens (métabolisme)</term><term>Transcriptome (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Phenazines</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Biological Control Agents</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phytophthora infestans</term><term>Pseudomonas fluorescens</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Phytophthora infestans</term><term>Phénazines</term><term>Pseudomonas fluorescens</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Profiling</term><term>Sequence Analysis, RNA</term><term>Transcriptome</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Agents de lutte biologique</term><term>Analyse de profil d'expression de gènes</term><term>Analyse de séquence d'ARN</term><term>Transcriptome</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Phytophthora infestans is responsible for late blight, one of the most important potato diseases. Phenazine-1-carboxylic acid (PCA)-producing Pseudomonas fluorescens strain LBUM223 isolated in our laboratory shows biocontrol potential against various plant pathogens. To characterize the effect of LBUM223 on the transcriptome of P. infestans, we conducted an in vitro time-course study. Confrontational assay was performed using P. infestans inoculated alone (control) or with LBUM223, its phzC- isogenic mutant (not producing PCA), or exogenically applied PCA. Destructive sampling was performed at 6, 9 and 12 days and the transcriptome of P. infestans was analysed using RNA-Seq. The expression of a subset of differentially expressed genes was validated by RT-qPCR.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Both LBUM223 and exogenically applied PCA significantly repressed P. infestans' growth at all times. Compared to the control treatment, transcriptomic analyses showed that the percentages of all P. infestans' genes significantly altered by LBUM223 and exogenically applied PCA increased as time progressed, from 50 to 61% and from to 32 to 46%, respectively. When applying an absolute cut-off value of 3 fold change or more for all three harvesting times, 207 genes were found significantly differentially expressed by PCA, either produced by LBUM223 or exogenically applied. Gene ontology analysis revealed that both treatments altered the expression of key functional genes involved in major functions like phosphorylation mechanisms, transmembrane transport and oxidoreduction activities. Interestingly, even though no host plant tissue was present in the in vitro system, PCA also led to the overexpression of several genes encoding effectors. The mutant only slightly repressed P. infestans' growth and barely altered its transcriptome.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>Our study suggests that PCA is involved in P. infestans' growth repression and led to important transcriptomic changes by both up- and down-regulating gene expression in P. infestans over time. Different metabolic functions were altered and many effectors were found to be upregulated, suggesting their implication in biocontrol.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29914352</PMID><DateCompleted><Year>2018</Year><Month>12</Month><Day>11</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>11</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2164</ISSN><JournalIssue CitedMedium="Internet"><Volume>19</Volume><Issue>1</Issue><PubDate><Year>2018</Year><Month>Jun</Month><Day>19</Day></PubDate></JournalIssue><Title>BMC genomics</Title><ISOAbbreviation>BMC Genomics</ISOAbbreviation></Journal><ArticleTitle>Transcriptome alteration in Phytophthora infestans in response to phenazine-1-carboxylic acid production by Pseudomonas fluorescens strain LBUM223.</ArticleTitle><Pagination><MedlinePgn>474</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s12864-018-4852-1</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Phytophthora infestans is responsible for late blight, one of the most important potato diseases. Phenazine-1-carboxylic acid (PCA)-producing Pseudomonas fluorescens strain LBUM223 isolated in our laboratory shows biocontrol potential against various plant pathogens. To characterize the effect of LBUM223 on the transcriptome of P. infestans, we conducted an in vitro time-course study. Confrontational assay was performed using P. infestans inoculated alone (control) or with LBUM223, its phzC- isogenic mutant (not producing PCA), or exogenically applied PCA. Destructive sampling was performed at 6, 9 and 12 days and the transcriptome of P. infestans was analysed using RNA-Seq. The expression of a subset of differentially expressed genes was validated by RT-qPCR.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Both LBUM223 and exogenically applied PCA significantly repressed P. infestans' growth at all times. Compared to the control treatment, transcriptomic analyses showed that the percentages of all P. infestans' genes significantly altered by LBUM223 and exogenically applied PCA increased as time progressed, from 50 to 61% and from to 32 to 46%, respectively. When applying an absolute cut-off value of 3 fold change or more for all three harvesting times, 207 genes were found significantly differentially expressed by PCA, either produced by LBUM223 or exogenically applied. Gene ontology analysis revealed that both treatments altered the expression of key functional genes involved in major functions like phosphorylation mechanisms, transmembrane transport and oxidoreduction activities. Interestingly, even though no host plant tissue was present in the in vitro system, PCA also led to the overexpression of several genes encoding effectors. The mutant only slightly repressed P. infestans' growth and barely altered its transcriptome.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Our study suggests that PCA is involved in P. infestans' growth repression and led to important transcriptomic changes by both up- and down-regulating gene expression in P. infestans over time. Different metabolic functions were altered and many effectors were found to be upregulated, suggesting their implication in biocontrol.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Roquigny</LastName><ForeName>Roxane</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Biology, Université de Moncton, Moncton, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Novinscak</LastName><ForeName>Amy</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Biology, Université de Moncton, Moncton, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Arseneault</LastName><ForeName>Tanya</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Saint-Jean-sur-Richelieu Research and Development Center, Agriculture and Agri-Food Canada, Saint-Jean-sur-Richelieu, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Joly</LastName><ForeName>David L</ForeName><Initials>DL</Initials><AffiliationInfo><Affiliation>Department of Biology, Université de Moncton, Moncton, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Filion</LastName><ForeName>Martin</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Biology, Université de Moncton, Moncton, Canada. martin.filion@umoncton.ca.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>06</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>BMC Genomics</MedlineTA><NlmUniqueID>100965258</NlmUniqueID><ISSNLinking>1471-2164</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D061046">Biological Control Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010619">Phenazines</NameOfSubstance></Chemical><Chemical><RegistryNumber>2538-68-3</RegistryNumber><NameOfSubstance UI="C037165">1-phenazinecarboxylic acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D061046" MajorTopicYN="N">Biological Control Agents</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010619" MajorTopicYN="N">Phenazines</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011551" MajorTopicYN="N">Pseudomonas fluorescens</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017423" MajorTopicYN="N">Sequence Analysis, RNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D059467" MajorTopicYN="Y">Transcriptome</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Biocontrol</Keyword><Keyword MajorTopicYN="N">Phenazine-1-carboxylic acid</Keyword><Keyword MajorTopicYN="N">Phytophthora infestans</Keyword><Keyword MajorTopicYN="N">Pseudomonas fluorescens</Keyword><Keyword MajorTopicYN="N">Transcriptomics</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>12</Month><Day>12</Day></PubMedPubDate><PubMedPubDate 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Jul;8(7):e1002784</Citation><ArticleIdList><ArticleId IdType="pubmed">22792073</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 2007 Aug;10(4):358-65</Citation><ArticleIdList><ArticleId IdType="pubmed">17611143</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2009 Sep 17;461(7262):393-8</Citation><ArticleIdList><ArticleId IdType="pubmed">19741609</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2013 Feb 16;14:106</Citation><ArticleIdList><ArticleId IdType="pubmed">23414203</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2010 Jan 1;26(1):139-40</Citation><ArticleIdList><ArticleId IdType="pubmed">19910308</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013;8(3):e59517</Citation><ArticleIdList><ArticleId IdType="pubmed">23536880</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2010 Dec 09;11:700</Citation><ArticleIdList><ArticleId IdType="pubmed">21143935</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 1999 Apr 16;449(1):17-22</Citation><ArticleIdList><ArticleId IdType="pubmed">10225419</ArticleId></ArticleIdList></Reference><Reference><Citation>Pestic Biochem Physiol. 2015 Jan;117:39-46</Citation><ArticleIdList><ArticleId IdType="pubmed">25619910</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2015 Mar;105(3):342-9</Citation><ArticleIdList><ArticleId IdType="pubmed">25226526</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Methods. 2008 Jul;5(7):621-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18516045</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2008 Jun;36(10):3420-35</Citation><ArticleIdList><ArticleId IdType="pubmed">18445632</ArticleId></ArticleIdList></Reference><Reference><Citation>Molecules. 2016 May 11;21(5):</Citation><ArticleIdList><ArticleId IdType="pubmed">27187325</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2010 Nov 16;11:637</Citation><ArticleIdList><ArticleId IdType="pubmed">21080964</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013 Nov 11;8(11):e79042</Citation><ArticleIdList><ArticleId IdType="pubmed">24244413</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2009 Nov;10(6):795-803</Citation><ArticleIdList><ArticleId IdType="pubmed">19849785</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Chem Biol. 2011 Feb;15(1):149-55</Citation><ArticleIdList><ArticleId IdType="pubmed">21115388</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Biochem Biophys. 2017 Mar 1;617:101-105</Citation><ArticleIdList><ArticleId IdType="pubmed">27665998</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2009 Jan;37(1):1-13</Citation><ArticleIdList><ArticleId IdType="pubmed">19033363</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2006 Nov 16;444(7117):323-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17108957</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Signal. 2014 Jan 21;7(309):ra8</Citation><ArticleIdList><ArticleId IdType="pubmed">24448648</ArticleId></ArticleIdList></Reference><Reference><Citation>Antimicrob Agents Chemother. 1986 Mar;29(3):488-95</Citation><ArticleIdList><ArticleId IdType="pubmed">3087284</ArticleId></ArticleIdList></Reference><Reference><Citation>Gene. 2014 Mar 10;537(2):312-21</Citation><ArticleIdList><ArticleId IdType="pubmed">24361203</ArticleId></ArticleIdList></Reference><Reference><Citation>Brief Bioinform. 2013 Nov;14(6):671-83</Citation><ArticleIdList><ArticleId IdType="pubmed">22988256</ArticleId></ArticleIdList></Reference><Reference><Citation>J Bacteriol. 1988 Aug;170(8):3499-508</Citation><ArticleIdList><ArticleId IdType="pubmed">2841289</ArticleId></ArticleIdList></Reference><Reference><Citation>FEMS Microbiol Ecol. 2011 Jan;75(1):173-83</Citation><ArticleIdList><ArticleId IdType="pubmed">21073487</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2017 Oct 10;18(1):764</Citation><ArticleIdList><ArticleId IdType="pubmed">29017458</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechniques. 2003 Feb;34(2):374-8</Citation><ArticleIdList><ArticleId IdType="pubmed">12613259</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2006;57(9):1991-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16690625</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Phytopathol. 2006;44:417-45</Citation><ArticleIdList><ArticleId IdType="pubmed">16719720</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Biol. 2003;4(5):P3</Citation><ArticleIdList><ArticleId IdType="pubmed">12734009</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Immunol. 2005 Jun;6(6):587-92</Citation><ArticleIdList><ArticleId IdType="pubmed">15864310</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2017 Mar;107(3):273-279</Citation><ArticleIdList><ArticleId IdType="pubmed">27827009</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biosci Bioeng. 2013 Nov;116(5):580-4</Citation><ArticleIdList><ArticleId IdType="pubmed">23727350</ArticleId></ArticleIdList></Reference><Reference><Citation>FEMS Microbiol Lett. 2007 Jan;266(1):65-74</Citation><ArticleIdList><ArticleId IdType="pubmed">17083369</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Phytopathol. 2006;44:41-60</Citation><ArticleIdList><ArticleId IdType="pubmed">16448329</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2016 Dec 9;12 (12 ):e1006097</Citation><ArticleIdList><ArticleId IdType="pubmed">27936244</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2008 May;9(3):385-402</Citation><ArticleIdList><ArticleId IdType="pubmed">18705878</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2015 Oct;105(10):1311-7</Citation><ArticleIdList><ArticleId IdType="pubmed">25961336</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Biol. 2013 Jun 28;14(6):211</Citation><ArticleIdList><ArticleId IdType="pubmed">23809564</ArticleId></ArticleIdList></Reference><Reference><Citation>J Proteome Res. 2014 Apr 4;13(4):1848-59</Citation><ArticleIdList><ArticleId IdType="pubmed">24588563</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2017 Feb 23;18(1):198</Citation><ArticleIdList><ArticleId IdType="pubmed">28228125</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2009;4(1):44-57</Citation><ArticleIdList><ArticleId IdType="pubmed">19131956</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Enzymol. 2006;411:134-93</Citation><ArticleIdList><ArticleId IdType="pubmed">16939790</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2017 Feb 03;8:99</Citation><ArticleIdList><ArticleId IdType="pubmed">28217133</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2013 Oct;103(10):995-1000</Citation><ArticleIdList><ArticleId IdType="pubmed">23883153</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2009 Nov;10(6):843-55</Citation><ArticleIdList><ArticleId IdType="pubmed">19849790</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbiol Res. 2015 Jan;170:229-34</Citation><ArticleIdList><ArticleId IdType="pubmed">24985092</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Plant Biol. 2011 Aug;14(4):407-14</Citation><ArticleIdList><ArticleId IdType="pubmed">21641854</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Announc. 2015 May 07;3(3):null</Citation><ArticleIdList><ArticleId IdType="pubmed">25953163</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2012 Feb;78(3):804-12</Citation><ArticleIdList><ArticleId IdType="pubmed">22138981</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Roquigny, Roxane" sort="Roquigny, Roxane" uniqKey="Roquigny R" first="Roxane" last="Roquigny">Roxane Roquigny</name></noRegion><name sortKey="Arseneault, Tanya" sort="Arseneault, Tanya" uniqKey="Arseneault T" first="Tanya" last="Arseneault">Tanya Arseneault</name><name sortKey="Filion, Martin" sort="Filion, Martin" uniqKey="Filion M" first="Martin" last="Filion">Martin Filion</name><name sortKey="Joly, David L" sort="Joly, David L" uniqKey="Joly D" 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